The course overviews methods for isolation and purification of organic compounds on a semi- and preparative scale as well as most widely used analytic methods for the determination of purity of organic compounds. The necessity for the pure compounds per se, and for the information on their purity is discussed.
Most common methods for fractionation are overviewed: extraction, crystallization, distillation, sublimation, centrifugation. Application of modern analytical methods (chromatography, mass spectrometry, NMR, UV, and IR spectroscopies) to quantitative and qualitative analyses is overviewed.