The course "Modelling and Simulation in Logistics" is aimed at overviewing simulation application for analysis and operation improvement of logistic systems. Different simulation approaches to modeling logistic systems are introduced, including continuous and discrete-event simulation and systems dynamics. Basics of complex systems discrete-event simulation are given, as well as its application to analysis and design of logistic systems. Some examples of simulation-based analysis and operation improvement of logistic systems are discussed, including practical experiences of Latvian companies. Practical skills in realization of acquired simulation methods are provided on the basis of modern simulation software (for example, Simul8).